Pharmacokinetics and bioavailability of liensinine in mouse blood by UPLC-MS/MS

Abstract Liensinine is a bisbenzyltetrahydroisoquinoline alkaloid extracted from lotus ( Nelumbo nucifera GAERTNER., Nelumbonaceae), especially in its embryo loti “Lien Tze Hsin” (green of mature seed). A rapid and simple UPLC-MS/MS method was developed to determine liensinine mouse blood application pharmacokinetic study. The samples were preprocessed by protein precipitation using acetonitrile. Midazolam (internal standard, IS) gradient eluted mobile phase methanol water (0.1% formic acid) Waters UPLC BEH C18 column. multiple reaction monitoring m / z 611.3 → 206.1 for 326.2 291.1 IS with an electrospray ionization (ESI) source used quantitative detection. calibration curve ranged 0.5 400 ng/mL r > 0.995). accuracy 92.2 108.2%, the precision intra-day inter-day less than 14%, matrix effect between 100.0% 109.6%, recovery better 71.0%. successfully study mice after oral (5 mg/kg) intravenous administration (1 mg/kg), absolute availability 1.8%.